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Human IL1 alpha protein (active)

Human IL1 alpha protein (active). GTX00264-pro
Human IL1 alpha protein (active). GTX00264-pro
Human IL1 alpha protein (active). GTX00264-pro

Cat No. GTX00264-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

Cell proliferation of Jurkat cells after stimulated with IL1a. Interleukin 1 alpha (IL1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. To test the effect of IL1a on cell proliferation, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well with 2% serum standard RPMI-1640 including various concentrations of recombinant human IL1a. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Jurkat cells after incubation with IL1a for 96h observed by inverted microscope.

Observed MW

24 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu98~Ala271 (NP_000566.3)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 1 alpha,IL-1A,IL1,IL1-ALPHA,IL1F1

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL1 alpha protein (active). GTX00264-pro

GTX00264-pro Functional Assay Image

Cell proliferation effect of GTX00264-pro Human IL1 alpha protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Jurkat cells cultured in RPMI-1640 for 96hrs.
(B) Jurkat cells cultured in RPMI-1640, stimulated with 10 ng/ml IL1 alpha for 96hrs.

Human IL1 alpha protein (active). GTX00264-pro

GTX00264-pro Image

WB analysis of GTX00264-pro Human IL1 alpha protein (active).

Human IL1 alpha protein (active). GTX00264-pro

GTX00264-pro Image

SDS-PAGE analysis of GTX00264-pro Human IL1 alpha protein (active).

REFERENCE
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