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Human IL1 beta protein, His tag (active)

Human IL1 beta protein, His tag (active). GTX00115-pro
Human IL1 beta protein, His tag (active). GTX00115-pro
Human IL1 beta protein, His tag (active). GTX00115-pro

Cat No. GTX00115-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($289)
See all IL1 beta protein products

APPLICATION

Application Note

Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1×10⁵ cells/ml, and allowed to attach overnight before treated with or without certain concentrations (1 ng/ml, 10 ng/ml) of IL1-β for 4h. IL-6 levels in the cell supernatant were determined by ELISA that IL-6 increased significantly after stimulated with IL1-β.

Observed MW

22 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala117~Ser269 (NP_000567.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human IL1 beta protein, His tag (active). GTX00115-pro

GTX00115-pro Functional Assay Image

ELISA detection of secreted IL-6 protein activation from A549 cells induced by GTX00115-pro Human IL1 beta protein (active) for 4hrs.
Recombinant protein concentrations: 1, and 10 ng/ml.

Human IL1 beta protein, His tag (active). GTX00115-pro

GTX00115-pro Image

SDS-PAGE analysis of GTX00115-pro Human IL1 beta protein (active).

Human IL1 beta protein, His tag (active). GTX00115-pro

GTX00115-pro Image

WB analysis of GTX00115-pro Human IL1 beta protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 289