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Human IL10 Receptor beta protein, His tag

Human IL10 Receptor beta protein, His tag. GTX00131-pro
Human IL10 Receptor beta protein, His tag. GTX00131-pro
Human IL10 Receptor beta protein, His tag. GTX00131-pro

Cat. No. GTX00131-pro

Reference 1

Application

 Functional Assay

Species

 Human
Package
10 μg ($259)
See all IL10 Receptor beta protein products

APPLICATION

Application Note

IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL10-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was in a dose dependent manner.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu87~Ala243 (NP_000619.3)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 10 receptor subunit beta , CDW210B , CRF2-4 , CRFB4 , D21S58 , D21S66 , IL-10R2

Background

The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human IL10 Receptor beta protein, His tag. GTX00131-pro

GTX00131-pro Functional Assay Image

Functional ELISA analysis of GTX00131-pro Human IL10 Receptor beta protein which can bind immobilized IL10 protein.

Human IL10 Receptor beta protein, His tag. GTX00131-pro

GTX00131-pro Image

SDS-PAGE analysis of GTX00131-pro Human IL10 Receptor beta protein.

Human IL10 Receptor beta protein, His tag. GTX00131-pro

GTX00131-pro Image

WB analysis of GTX00131-pro Human IL10 Receptor beta protein.

REFERENCE

Application Reference

Wu Y et al. Exp Ther Med 2020; 20 (2) : 1012-1020 Interleukin-22 is elevated in the atrium and plasma of patients with atrial fibrillation and increases collagen synthesis in transforming growth factor-β1-treated cardiac fibroblasts via the JNK pathway.
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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 259