APPLICATION
Application Note
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ made by cells such as macrophages and Th1 T cells. Therefore, THP-1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1 μg/ml) in the absence or presence of IL-10 (5 ng/ml, 10 ng/ml) for 24h and production of IL-1β was determined in the supernatants by cytokine specific ELISA. Activation of THP-1 cells with LPS (1 μg/ml) resulted in high levels of production of IL-1β and strong inhibitory effects of IL-10 (5 ng/ml, 10 ng/ml) were observed on the production of IL-1β.
Observed MW
18 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Ser19~Asn178 (NP_000563.1)
Expression System
HEK293 cells
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF
Background
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Database
Research Area
DATA IMAGES
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GTX00086-pro Functional Assay Image
ELISA detection of secreted IL-1β protein expression from 1 μg/ml LPS mediated THP-1 cells reduced by GTX00086-pro Human IL10 protein (active).
Recombinant protein concentrations: 5, and 10 ng/ml.
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GTX00086-pro Image
WB analysis of GTX00086-pro Human IL10 protein (active).
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GTX00086-pro Image
SDS-PAGE analysis of GTX00086-pro Human IL10 protein (active).
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REFERENCE
There are currently no references for Human IL10 protein, His tag (active) (GTX00086-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human IL10 protein, His tag (active) (GTX00086-pro). Be the first to share your experience with this product.
