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Human IL11 protein (active)

Human IL11 protein (active). GTX00087-pro
Human IL11 protein (active). GTX00087-pro
Human IL11 protein (active). GTX00087-pro

Cat No. GTX00087-pro

Application

Functional Assay

Species

Human
Package
10 μg ($289)

APPLICATION

Application Note

IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 1x10⁴ cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope.

Observed MW

27 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro22~Leu199 (NP_000632.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 11 , AGIF , IL-11

Background

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Human IL11 protein (active). GTX00087-pro

GTX00087-pro Functional Assay Image

Cell proliferation effect of GTX00087-pro Human IL11 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Jurkat cells cultured in RPMI-1640 for 72hrs.
(B) Jurkat cells cultured in RPMI-1640, stimulated with 100 ng/ml IL11 for 72hrs.

Human IL11 protein (active). GTX00087-pro

GTX00087-pro Image

SDS-PAGE analysis of GTX00087-pro Human IL11 protein (active).

Human IL11 protein (active). GTX00087-pro

GTX00087-pro Image

WB analysis of GTX00087-pro Human IL11 protein (active).

REFERENCE

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REVIEW

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