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Human IL12 protein, His tag (active)

Human IL12 protein, His tag (active). GTX66877-pro

Cat No. GTX66877-pro

Application

Functional Assay

Species

Human
Package
50 μg ($289)

APPLICATION

Application Note

The activity is determined by the IFN-g ELISA in a using NK-92 cells. The ED₅₀ for this effect is less than or equal to 0.03 ng/ml.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

C-terminal His-Tag; Length: 203 a.a. (IL12A), 306 a.a. (IL12A), 509 a.a. (heterodimeric IL12). Sequence of IL12A: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH
Sequence of IL12B: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS

Expression System

Baculovirus

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 12A , interleukin 12B , CLMF , IL-12A , NFSK , NKSF1 , P35 , CLMF , CLMF2 , IL-12B , IMD28 , IMD29 , NKSF , NKSF2

Database

Research Area

DATA IMAGES

Human IL12 protein, His tag (active). GTX66877-pro

GTX66877-pro Image

3 μg of GTX66877-pro Human IL12 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 289