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Human IL15 protein, His tag (active)

Human IL15 protein, His tag (active). GTX00088-pro
Human IL15 protein, His tag (active). GTX00088-pro
Human IL15 protein, His tag (active). GTX00088-pro
Human IL15 protein, His tag (active). GTX00088-pro

Cat. No. GTX00088-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all IL15 protein products

APPLICATION

Application Note

Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 1x10⁴ cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope.

Observed MW

14/16/18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Trp50~Ser162 (NP_000576.1)

Expression System

HEK293 cells

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 15 , IL-15

Background

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Human IL15 protein, His tag (active). GTX00088-pro

GTX00088-pro Functional Assay Image

Cell proliferation effect of GTX00088-pro Human IL15 protein (active).
(A) Unstimulated Jurkat cells cultured in RPMI-1640 for 72hrs.
(B) Jurkat cells cultured in RPMI-1640, stimulated with 1 ng/ml IL15 for 72hrs.

Human IL15 protein, His tag (active). GTX00088-pro

GTX00088-pro Functional Assay Image

The dose effect curve of GTX00088-pro Human IL15 protein (active) on Jurkat cells. After incubation of Human IL15 protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human IL15 protein, His tag (active). GTX00088-pro

GTX00088-pro Image

SDS-PAGE analysis of GTX00088-pro Human IL15 protein (active).

Human IL15 protein, His tag (active). GTX00088-pro

GTX00088-pro Image

WB analysis of GTX00088-pro Human IL15 protein (active).

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SDS
Proclin.pdf
Package List Price ($)
$ 309