APPLICATION
Application Note
IL17RC (Interleukin-17 receptor C) is a receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. This protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. A binding ELISA assay was conducted to detect the association of IL17RC with IL17RA. Briefly, IL17RC were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17RC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RC with IL17RA was in a dose dependent manner.
Observed MW
26 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Asn257~Asn477① (NP_001190192.1)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 17 receptor C , CANDF9 , IL17-RL , IL17RL
Background
This gene encodes a single-pass type I membrane protein that shares similarity with the interleukin-17 receptor (IL-17RA). Unlike IL-17RA, which is predominantly expressed in hemopoietic cells, and binds with high affinity to only IL-17A, this protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. The proinflammatory cytokines, IL-17A and IL-17F, have been implicated in the progression of inflammatory and autoimmune diseases. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, and it has been proposed that soluble, secreted proteins lacking transmembrane and intracellular domains may function as extracellular antagonists to cytokine signaling. [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX00143-pro Functional Assay Image
Functional ELISA analysis of GTX00143-pro Human IL17 Receptor C protein which can bind immobilized IL17RA protein.
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GTX00143-pro Image
SDS-PAGE analysis of GTX00143-pro Human IL17 Receptor C protein.
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GTX00143-pro Image
WB analysis of GTX00143-pro Human IL17 Receptor C protein.
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REFERENCE
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REVIEW
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