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Human IL17 Receptor D protein (active)

Human IL17 Receptor D protein (active). GTX00142-pro
Human IL17 Receptor D protein (active). GTX00142-pro
Human IL17 Receptor D protein (active). GTX00142-pro
Human IL17 Receptor D protein (active). GTX00142-pro

Cat No. GTX00142-pro

Application

Functional Assay

Species

Human
Package
10 μg ($329)

APPLICATION

Application Note

IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in images respectively and this effect was in a dose dependent manner.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Thr157~Arg299 (NP_001305793.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17 receptor D , HH18 , IL-17RD , IL17RLM , SEF

Background

This gene encodes a membrane protein belonging to the interleukin-17 receptor (IL-17R) protein family. The encoded protein is a component of the interleukin-17 receptor signaling complex, and the interaction between this protein and IL-17R does not require the interleukin. The gene product also affects fibroblast growth factor signaling, inhibiting or stimulating growth through MAPK/ERK signaling. Alternate splicing generates multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Human IL17 Receptor D protein (active). GTX00142-pro

GTX00142-pro Functional Assay Image

Functional ELISA analysis of GTX00142-pro Human IL17 Receptor D protein (active) which can bind immobilized IL17 protein.

Human IL17 Receptor D protein (active). GTX00142-pro

GTX00142-pro Functional Assay Image

Functional ELISA analysis of GTX00142-pro Human IL17 Receptor D protein (active) which can bind immobilized IL17RA protein.

Human IL17 Receptor D protein (active). GTX00142-pro

GTX00142-pro Image

SDS-PAGE analysis of GTX00142-pro Human IL17 Receptor D protein (active).

Human IL17 Receptor D protein (active). GTX00142-pro

GTX00142-pro Image

WB analysis of GTX00142-pro Human IL17 Receptor D protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 329