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Human IL17 Receptor alpha protein (active)

Human IL17 Receptor alpha protein (active). GTX00132-pro
Human IL17 Receptor alpha protein (active). GTX00132-pro
Human IL17 Receptor alpha protein (active). GTX00132-pro

Cat No. GTX00132-pro

Application

Functional Assay

Species

Human
Package
10 μg ($249)

APPLICATION

Application Note

Interleukin-17 receptor A (IL17RA) is a cytokine receptor which binds interleukin 17A (IL17A). IL17A is a proinflammatory cytokine secreted by activated T-lymphocytes. IL17RA, as a transmembrane protein, is a ubiquitous type I membrane glycoprotein that binds IL17A, IL17F and IL17C. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RA with recombinant human IL17A. Briefly, IL17RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17A-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RA with IL17A was in a dose dependent manner.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro377~Phe534 (NP_001276834.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17 receptor A , CANDF5 , CD217 , CDw217 , IL-17RA , IL17R , IMD51 , hIL-17R

Background

Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Human IL17 Receptor alpha protein (active). GTX00132-pro

GTX00132-pro Functional Assay Image

Functional ELISA analysis of GTX00132-pro Human IL17 Receptor alpha protein (active) which can bind immobilized IL17 protein.

Human IL17 Receptor alpha protein (active). GTX00132-pro

GTX00132-pro Image

SDS-PAGE analysis of GTX00132-pro Human IL17 Receptor alpha protein (active).

Human IL17 Receptor alpha protein (active). GTX00132-pro

GTX00132-pro Image

WB analysis of GTX00132-pro Human IL17 Receptor alpha protein (active).

REFERENCE

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REVIEW

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