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Human IL17 Receptor alpha protein (active)

Human IL17 Receptor alpha protein (active). GTX00132-pro
Human IL17 Receptor alpha protein (active). GTX00132-pro
Human IL17 Receptor alpha protein (active). GTX00132-pro

Cat No. GTX00132-pro

Application Functional Assay
Species Human
Package
10 μg ($249)
APPLICATION

Application Note

Interleukin-17 receptor A (IL17RA) is a cytokine receptor which binds interleukin 17A (IL17A). IL17A is a proinflammatory cytokine secreted by activated T-lymphocytes. IL17RA, as a transmembrane protein, is a ubiquitous type I membrane glycoprotein that binds IL17A, IL17F and IL17C. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RA with recombinant human IL17A. Briefly, IL17RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17A-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RA with IL17A was in a dose dependent manner.

Observed MW

18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro377~Phe534 (NP_001276834.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 17 receptor A,CANDF5,CD217,CDw217,IL-17RA,IL17R,IMD51,hIL-17R

Background

Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2014]

Database

DATA IMAGES
Human IL17 Receptor alpha protein (active). GTX00132-pro

GTX00132-pro Functional Assay Image

Functional ELISA analysis of GTX00132-pro Human IL17 Receptor alpha protein (active) which can bind immobilized IL17 protein.

Human IL17 Receptor alpha protein (active). GTX00132-pro

GTX00132-pro Image

SDS-PAGE analysis of GTX00132-pro Human IL17 Receptor alpha protein (active).

Human IL17 Receptor alpha protein (active). GTX00132-pro

GTX00132-pro Image

WB analysis of GTX00132-pro Human IL17 Receptor alpha protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 249