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Human IL17 Receptor beta protein (active)

Human IL17 Receptor beta protein (active). GTX00144-pro
Human IL17 Receptor beta protein (active). GTX00144-pro
Human IL17 Receptor beta protein (active). GTX00144-pro

Cat No. GTX00144-pro

Application

Functional Assay

Species

Human
Package
10 μg ($239)

APPLICATION

Application Note

IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was in a dose dependent manner.

Observed MW

19 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp61~Thr200 (NP_061195.2)

Expression System

E. coli

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17 receptor B , CRL4 , EVI27 , IL17BR , IL17RH1

Background

The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E, but does not bind to IL17 and IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. The expression of the rat counterpart of this gene was found to be significantly up-regulated during intestinal inflammation, which suggested the immunoregulatory activity of this receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human IL17 Receptor beta protein (active). GTX00144-pro

GTX00144-pro Functional Assay Image

Functional ELISA analysis of GTX00144-pro Human IL17 Receptor beta protein (active) which can bind immobilized IL17B protein.

Human IL17 Receptor beta protein (active). GTX00144-pro

GTX00144-pro Image

WB analysis of GTX00144-pro Human IL17 Receptor beta protein (active).

Human IL17 Receptor beta protein (active). GTX00144-pro

GTX00144-pro Image

SDS-PAGE analysis of GTX00144-pro Human IL17 Receptor beta protein (active).

REFERENCE

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REVIEW

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