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Human IL17 Receptor beta protein (active)

Human IL17 Receptor beta protein (active). GTX00144-pro
Human IL17 Receptor beta protein (active). GTX00144-pro
Human IL17 Receptor beta protein (active). GTX00144-pro

Cat No. GTX00144-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was in a dose dependent manner.

Observed MW

19 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp61~Thr200 (NP_061195.2)

Expression System

E. coli

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 17 receptor B,CRL4,EVI27,IL17BR,IL17RH1

Background

The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E, but does not bind to IL17 and IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. The expression of the rat counterpart of this gene was found to be significantly up-regulated during intestinal inflammation, which suggested the immunoregulatory activity of this receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL17 Receptor beta protein (active). GTX00144-pro

GTX00144-pro Functional Assay Image

Functional ELISA analysis of GTX00144-pro Human IL17 Receptor beta protein (active) which can bind immobilized IL17B protein.

Human IL17 Receptor beta protein (active). GTX00144-pro

GTX00144-pro Image

WB analysis of GTX00144-pro Human IL17 Receptor beta protein (active).

Human IL17 Receptor beta protein (active). GTX00144-pro

GTX00144-pro Image

SDS-PAGE analysis of GTX00144-pro Human IL17 Receptor beta protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239