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Human IL17A protein, His tag

Human IL17A protein, His tag. GTX00090-pro
Human IL17A protein, His tag. GTX00090-pro
Human IL17A protein, His tag. GTX00090-pro

Cat. No. GTX00090-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($219)
See all IL17A protein products

APPLICATION

Application Note

IL17 (interleukin 17) is a member of IL17 cytokine family, which is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. It is reported that IL17 is a ligand for IL17RA, indicating its interaction with IL17RA. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17 with recombinant human IL17RA. Briefly, IL17 were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17 with IL17RA was in a dose dependent manner.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile20~Ala155 (NP_002181.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17A , CTLA-8 , CTLA8 , IL-17 , IL-17A , IL17

Background

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human IL17A protein, His tag. GTX00090-pro

GTX00090-pro Functional Assay Image

Functional ELISA analysis of GTX00090-pro Human IL17A protein which can bind immobilized IL17RA protein.

Human IL17A protein, His tag. GTX00090-pro

GTX00090-pro Image

SDS-PAGE analysis of GTX00090-pro Human IL17A protein.

Human IL17A protein, His tag. GTX00090-pro

GTX00090-pro Image

WB analysis of GTX00090-pro Human IL17A protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 219