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Human IL17E protein (active)

Human IL17E protein (active). GTX00133-pro
Human IL17E protein (active). GTX00133-pro
Human IL17E protein (active). GTX00133-pro

Cat No. GTX00133-pro

Application

Functional Assay

Species

Human
Package
10 μg ($249)

APPLICATION

Application Note

IL25 (Interleukin-25), also known as IL17E, is reported to induce NF-κB activation, and stimulate the production of IL-8. Besides, this cytokine shares the sequence similarity with IL17, which is considered as a ligand for IL17RA. Thus, a binding ELISA assay was conducted to detect the association of recombinant human IL25 with IL17RA. Briefly, IL25 were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL25 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL25 with IL17RA was in a dose dependent manner.

Observed MW

21 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Met28~Gly177 (NP_073626.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 25 , IL17E

Background

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Database

DATA IMAGES

Human IL17E protein (active). GTX00133-pro

GTX00133-pro Functional Assay Image

Functional ELISA analysis of GTX00133-pro Human IL17E protein (active) which can bind immobilized IL17RA protein.

Human IL17E protein (active). GTX00133-pro

GTX00133-pro Image

WB analysis of GTX00133-pro Human IL17E protein (active).

Human IL17E protein (active). GTX00133-pro

GTX00133-pro Image

SDS-PAGE analysis of GTX00133-pro Human IL17E protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 249