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Human IL18 protein, His tag

Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro

Cat. No. GTX00227-pro

Application

 ELISA, Functional Assay

Species

 Human
Package
10 μg ($299)
See all IL18 protein products

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX637584, Detection : GTX637411 or Capture : GTX637411, Detection : GTX637584.
The detection antibody requires HRP conjugation. Please contact us for the conjugation service.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr37~Asp193 (NP_001230140.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 18 , IGIF , IL-18 , IL-1g , IL1F4

Background

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Functional Assay Image

Functional ELISA analysis of GTX00227-pro Human IL18 protein which can bind immobilized CASP1 protein.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL)
Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

SDS-PAGE analysis of GTX00227-pro Human IL18 protein.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

WB analysis of GTX00227-pro Human IL18 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299