Functional ELISA analysis of GTX00227-pro Human IL18 protein which can bind immobilized CASP1 protein.

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL2938] (GTX640322) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584) (1 μg/mL)
Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640322. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL)
Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

WB analysis of GTX00227-pro Human IL18 protein.

SDS-PAGE analysis of GTX00227-pro Human IL18 protein.