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Human IL18 protein (active)

Human IL18 protein (active). GTX00227-pro
Human IL18 protein (active). GTX00227-pro
Human IL18 protein (active). GTX00227-pro

Cat No. GTX00227-pro

Application

Functional Assay

Species

Human
Package
10 μg ($279)

APPLICATION

Application Note

Interleukin-18 (IL18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). Besides, Caspase 1 (CASP1) has been identified as an interactor of IL18, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL18 and recombinant human CASP1. Briefly, IL18 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL18 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL18 and CASP1 was in a dose dependent manner.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr37~Asp193 (NP_001230140.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 18 , IGIF , IL-18 , IL-1g , IL1F4

Background

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Human IL18 protein (active). GTX00227-pro

GTX00227-pro Functional Assay Image

Functional ELISA analysis of GTX00227-pro Human IL18 protein (active) which can bind immobilized CASP1 protein.

Human IL18 protein (active). GTX00227-pro

GTX00227-pro Image

WB analysis of GTX00227-pro Human IL18 protein (active).

Human IL18 protein (active). GTX00227-pro

GTX00227-pro Image

SDS-PAGE analysis of GTX00227-pro Human IL18 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 279