GeneTex
  • Country / Location Selection

United States (US)

Human IL2 protein (active)

Human IL2 protein (active). GTX00092-pro
Human IL2 protein (active). GTX00092-pro
Human IL2 protein (active). GTX00092-pro
Human IL2 protein (active). GTX00092-pro

Cat No. GTX00092-pro

Application

Functional Assay

Species

Human
Package
10 μg ($259)

APPLICATION

Application Note

IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly,   CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h,   cells were observed by inverted microscope. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope.

Observed MW

18 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.6) with 5% trehalose. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala21~Thr153 (NP_000577.2)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 , IL-2 , TCGF , lymphokine

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human IL2 protein (active). GTX00092-pro

GTX00092-pro Functional Assay Image

Cell proliferation effect of GTX00092-pro Human IL2 protein (active).
(A) Unstimulated CTLL-2 cells cultured in RPMI-1640 for 48hrs.
(B) CTLL-2 cells cultured in RPMI-1640, stimulated with 10 ng/ml IL2 for 48hrs.

Human IL2 protein (active). GTX00092-pro

GTX00092-pro Functional Assay Image

The dose effect curve of GTX00092-pro Human IL2 protein (active) on CTLL-2 cells. After incubation of Human IL2 protein (active) for48 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human IL2 protein (active). GTX00092-pro

GTX00092-pro Image

WB analysis of GTX00092-pro Human IL2 protein (active).

Human IL2 protein (active). GTX00092-pro

GTX00092-pro Image

SDS-PAGE analysis of GTX00092-pro Human IL2 protein (active).

REFERENCE

There are currently no references for Human IL2 protein (active) (GTX00092-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Human IL2 protein (active) (GTX00092-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 259