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Human IL20 protein (active)

Human IL20 protein (active). GTX00137-pro
Human IL20 protein (active). GTX00137-pro
Human IL20 protein (active). GTX00137-pro

Cat No. GTX00137-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly,  ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard RPMI-1640   prior to the addition of various concentrations of IL20. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The human IL20 significantly decreased cell viability of ECV-304 cells.

Observed MW

21 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu25~Glu176 (NP_061194.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 20,IL-20,IL10D,ZCYTO10

Background

The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Human IL20 protein (active). GTX00137-pro

GTX00137-pro Functional Assay Image

Cell proliferation effect of GTX00137-pro Human IL20 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated ECV-304 cells cultured in RPMI-1640 for 48hrs.
(B) ECV-304 cells cultured in RPMI-1640, stimulated with 500 ng/ml IL20 for 48hrs.

Human IL20 protein (active). GTX00137-pro

GTX00137-pro Image

SDS-PAGE analysis of GTX00137-pro Human IL20 protein (active).

Human IL20 protein (active). GTX00137-pro

GTX00137-pro Image

WB analysis of GTX00137-pro Human IL20 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239