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Human IL29 protein (active)

Human IL29 protein (active). GTX00245-pro
Human IL29 protein (active). GTX00245-pro
Human IL29 protein (active). GTX00245-pro

Cat No. GTX00245-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Interleukin-29 (IL-29) is a member of the helical cytokine family and is a type III interferon. It is also known as IFNλ1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. Besides, ATPase, Ca Transporting, Plasma Membrane 2 (ATP2B2) has been identified as an interactor of IL29, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL29 and recombinant human ATP2B2. Briefly, IL29 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ATP2B2-coated microtiter wells and incubated for 2h at 37 ºC. Wells were washed with PBST and incubated for 1h with anti-IL29 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL29 and ATP2B2 was in a dose dependent manner.

Observed MW

27 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly20~Thr200 (NP_742152.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interferon lambda 1 , IL-29 , IL29

Background

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL29 protein (active). GTX00245-pro

GTX00245-pro Functional Assay Image

Functional ELISA analysis of GTX00245-pro Human IL29 protein (active) which can bind immobilized ATP2B2 protein.

Human IL29 protein (active). GTX00245-pro

GTX00245-pro Image

SDS-PAGE analysis of GTX00245-pro Human IL29 protein (active).

Human IL29 protein (active). GTX00245-pro

GTX00245-pro Image

WB analysis of GTX00245-pro Human IL29 protein (active).

REFERENCE
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REVIEW
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