APPLICATION
Application Note
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED₅₀ range ≤ 0.5 ng/ml.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS (pH7.4), 10% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1 week), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
His-Tagged, 25-153aa (P05112, NP_000580)
Expression System
HEK293 cells
Purity
> 90% by SDS-PAGE
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 4 , BCGF-1 , BCGF1 , BSF-1 , BSF1 , IL-4
Background
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX02862-pro Image
SDS-PAGE analysis of 3μg GTX02862-pro Human IL4 protein, His tag (active) under reducing condition.
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GTX02862-pro Functional Assay Image
Cell proliferation assay of Human IL4 protein (active) (GTX02862-pro) in TF-1 cells. ED₅₀ : ≤ 0.5 ng/ml
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REFERENCE
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REVIEW
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