APPLICATION
Application Note
Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells and monocyte. As reported, IL-4 would induce the differentiation of THP-1 cells into dendritic cells and macrophages in vitro. THP-1 cells were cultured in RPMI-1640 and stimulated with 2 ng/ml IL-4, after 12 days of stimulation, cell bodies enlarged with extending pseudopodia, and vesicular bodies appeared within the cells, which showed a morphological characteristics of dendritic cells and macrophages.
Observed MW
20 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; His25~Ser153 (NP_000580.1)
Expression System
HEK293 cells
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 4 , BCGF-1 , BCGF1 , BSF-1 , BSF1 , IL-4
Background
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00093-pro Functional Assay Image
Cell differentiation effect of GTX00093-pro Human IL4 protein (active).
(A) Unstimulated THP-1 cells cultured in RPMI-1640.
(B) THP-1 cells cultured in RPMI-1640, stimulated with IL-4.
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GTX00093-pro Image
WB analysis of GTX00093-pro Human IL4 protein (active).
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GTX00093-pro Image
SDS-PAGE analysis of GTX00093-pro Human IL4 protein (active).
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REFERENCE
There are currently no references for Human IL4 protein, His tag (active) (GTX00093-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human IL4 protein, His tag (active) (GTX00093-pro). Be the first to share your experience with this product.
