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Human IL4 protein (active)

Human IL4 protein (active). GTX00093-pro
Human IL4 protein (active). GTX00093-pro
Human IL4 protein (active). GTX00093-pro

Cat No. GTX00093-pro

Application Functional Assay
Species Human
Package
10 μg ($269)
See all IL4 protein products
APPLICATION

Application Note

Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells and monocyte. As reported, IL-4 would induce the differentiation of THP-1 cells into dendritic cells and macrophages in vitro. THP-1 cells were cultured in RPMI-1640 and stimulated with 2 ng/ml IL-4, after 12 days of stimulation, cell bodies enlarged with extending pseudopodia, and vesicular bodies appeared within the cells, which showed a morphological characteristics of dendritic cells and macrophages.

Observed MW

20 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; His25~Ser153 (NP_000580.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 4,BCGF-1,BCGF1,BSF-1,BSF1,IL-4

Background

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL4 protein (active). GTX00093-pro

GTX00093-pro Functional Assay Image

Cell differentiation effect of GTX00093-pro Human IL4 protein (active).
(A) Unstimulated THP-1 cells cultured in RPMI-1640.
(B) THP-1 cells cultured in RPMI-1640, stimulated with IL-4.
Human IL4 protein (active). GTX00093-pro

GTX00093-pro Image

SDS-PAGE analysis of GTX00093-pro Human IL4 protein (active).
Human IL4 protein (active). GTX00093-pro

GTX00093-pro Image

WB analysis of GTX00093-pro Human IL4 protein (active).
REFERENCE
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Package List Price ($)
$ 269