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Human IL6 protein, His tag

Human IL6 protein, His tag. GTX135982-pro
Human IL6 protein, His tag. GTX135982-pro
Human IL6 protein, His tag. GTX135982-pro
Human IL6 protein, His tag. GTX135982-pro

Cat. No. GTX135982-pro

Application

ELISA, Sandwich ELISA

Species

Human
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX636571, Detection : GTX636473 or Capture : GTX636573, Detection : GTX636473.
Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal His tagged Human IL-6 protein of NP_000591.1.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Human IL6 protein, His tag. GTX135982-pro

GTX135982-pro ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)

Human IL6 protein, His tag. GTX135982-pro

GTX135982-pro ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)

Human IL6 protein, His tag. GTX135982-pro

GTX135982-pro ELISA Image

Sandwich ELISA detection of recombinant IL6 protein, His tag (GTX135982-pro) using IL6 antibody as capture antibody at concentration of 5 μg/mL and biotinylated IL6 antibody as detection antibody at concentration of 1 μg/mL. Peroxidase Streptavidin was diluted at 1:10000 and used to detect the primary antibody.

Human IL6 protein, His tag. GTX135982-pro

GTX135982-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (1000-15.625 ng/mL). Coated protein was probed with IL-6 antibody (GTX135982) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 499