Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

Sandwich ELISA detection of Human IL6 protein, His tag (GTX135982-pro) using ELISA pairs below.
Pair 1:
Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)
Pair 2:
Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)

Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.