APPLICATION
Application Note
Recommended antibody pairs for sandwich ELISA:
Capture : GTX636571 / GTX636573, Detection : GTX636473 / GTX638598.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal His tagged Human IL-6 protein of NP_000591.1.
Expression System
HEK293 cells
Purity
>95%
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6
Cellular Localization
Secreted
Background
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
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GTX135982-pro ELISA Image
Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)
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GTX135982-pro ELISA Image
Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)
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GTX135982-pro ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX135982-pro ELISA Image
Sandwich ELISA detection of Human IL6 protein, His tag (GTX135982-pro) using ELISA pairs below. Pair 1:Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL) Pair 2:Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)
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REFERENCE
There are currently no references for Human IL6 protein, His tag (GTX135982-pro). Be the first to share your publications with this product.
REVIEW
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