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Human IL6 protein (active)

Human IL6 protein (active). GTX00095-pro
Human IL6 protein (active). GTX00095-pro
Human IL6 protein (active). GTX00095-pro
Human IL6 protein (active). GTX00095-pro

Cat No. GTX00095-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Interleukin 6  (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Current data suggest that direct application of IL-6 on breast cancer cells inhibits proliferation in ER-positive (estrogen- receptor- positive) cells through the Jak/Stat3 pathway. To test the inhibitory effect of IL-6 on proliferation of ER-positive  MCF-7 cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM   prior to the addition of various concentrations of IL-6. After incubated for 96h,  cells were observed by inverted microscope. The inhibitory effect of IL-6 on cell proliferation of MCF-7 cells observed by inverted microscope.

Observed MW

22/24 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val30~Met212 (NP_000591.1)

Expression System

293F cell

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES
Human IL6 protein (active). GTX00095-pro

GTX00095-pro Functional Assay Image

The inhibition effect of GTX00095-pro Human IL6 protein (active) on cell proliferation.
(A) Unstimulated MCF-7 cells cultured in serum-free DMEM for 96hrs.
(B) MCF-7 cells cultured in serum-free DMEM, stimulated with 100 ng/ml IL-6 for 96hrs.

Human IL6 protein (active). GTX00095-pro

GTX00095-pro Functional Assay Image

Cell suppression effect of GTX00095-pro Human IL6 protein (active) on MCF-7 cells. After incubation of Human IL6 protein (active) for 96hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).

Human IL6 protein (active). GTX00095-pro

GTX00095-pro Image

SDS-PAGE analysis of GTX00095-pro Human IL6 protein (active).

Human IL6 protein (active). GTX00095-pro

GTX00095-pro Image

WB analysis of GTX00095-pro Human IL6 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279