APPLICATION
Application Note
Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Current data suggest that direct application of IL-6 on breast cancer cells inhibits proliferation in ER-positive (estrogen- receptor- positive) cells through the Jak/Stat3 pathway. To test the inhibitory effect of IL-6 on proliferation of ER-positive MCF-7 cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IL-6. After incubated for 96h, cells were observed by inverted microscope. The inhibitory effect of IL-6 on cell proliferation of MCF-7 cells observed by inverted microscope.
Observed MW
22/24 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Val30~Met212 (NP_000591.1)
Expression System
HEK293 cells
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6
Background
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
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GTX00095-pro Functional Assay Image
The inhibition effect of GTX00095-pro Human IL6 protein (active) on cell proliferation.
(A) Unstimulated MCF-7 cells cultured in serum-free DMEM for 96hrs.
(B) MCF-7 cells cultured in serum-free DMEM, stimulated with 100 ng/ml IL-6 for 96hrs.
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GTX00095-pro Functional Assay Image
Cell suppression effect of GTX00095-pro Human IL6 protein (active) on MCF-7 cells. After incubation of Human IL6 protein (active) for 96hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).
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GTX00095-pro Image
SDS-PAGE analysis of GTX00095-pro Human IL6 protein (active).
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GTX00095-pro Image
WB analysis of GTX00095-pro Human IL6 protein (active).
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REFERENCE
There are currently no references for Human IL6 protein, His tag (active) (GTX00095-pro). Be the first to share your publications with this product.
REVIEW
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