APPLICATION
Application Note
Inter Alpha-Globulin Inhibitor H4 (ITIH4) is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. ITIH4 has been detected only in liver, and it seems to be upregulated during surgical trauma. It may also play a role in liver development or regeneration. Besides, Growth Factor Receptor Bound Protein 2 (Grb2) has been identified as an interactor of ITIH4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ITIH4 and recombinant human Grb2. Briefly, ITIH4 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to
Grb2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ITIH4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ITIH4 and Grb2 was in a dose dependent manner.
Observed MW
33 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Ala683~Val928 (NP_001159921.1)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
inter-alpha-trypsin inhibitor heavy chain family member 4 , GP120 , H4P , IHRP , ITI-HC4 , ITIHL1 , PK-120 , PK120
Background
The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX00226-pro Functional Assay Image
Functional ELISA analysis of GTX00226-pro Human ITIH4 protein which can bind immobilized Grb2 protein.
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GTX00226-pro Image
WB analysis of GTX00226-pro Human ITIH4 protein.
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GTX00226-pro Image
SDS-PAGE analysis of GTX00226-pro Human ITIH4 protein.
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REFERENCE
There are currently no references for Human ITIH4 protein, His tag (GTX00226-pro). Be the first to share your publications with this product.
REVIEW
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