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Human IgG isotype control (AP)

Cat No. GTX35066

Host

Human

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA
Package
500 μl ($99)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

50mM Tris pH8.0, 1mM MgCl₂, 50% Glycerol

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Conjugation

Alkaline Phosphatase (AP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Human IgG isotype control , Human isotype control for IgG , Polyclonal Human IgG isotype control , Human IgG negative control , Negative control for Human IgG , Isotype control for Human IgG , Human negative control for IgG , Isotype control to Human IgG

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 99