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Human IgG1 kappa isotype control (AP)

Cat. No. GTX35069

Host

 Human

Clonality

 Monoclonal

Isotype

 IgG1

Application

 WB, ELISA
Package
500 μl ($119)
See all Human IgG1 kappa chain isotype control products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

50mM Tris pH8.0, 1mM MgCl₂, 50% Glycerol

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Conjugation

Alkaline Phosphatase (AP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Human IgG1 kappa negative control , monoclonal Human IgG1 isotype control , Human IgG1 negative control , Isotype control to Human IgG1 kappa , Hum , Isotype control for Human IgG1 kappa , Human IgG1 isotype control , Negative control for Human IgG1 kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 119