20 mM Tris-HCl buffer (pH 8.0) with 1 mM DTT, 30% glycerol, 0.1 M NaCl.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.25 mg/ml (Please refer to the vial label for the specific concentration.)
Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSMELITIL EKTVSPDRLE LEAAQKFLER AAVENLPTFL VELSRVLANP GNSQVARVAA GLQIKNSLTS KDPDIKAQYQ QRWLAIDANA RREVKNYVLQ TLGTETYRPS SASQCVAGIA CAEIPVNQWP ELIPQLVANV TNPNSTEHMK ESTLEAIGYI CQDIDPEQLQ DKSNEILTAI IQGMRKEEPS NNVKLAATNA LLNSLEFTKA NFDKESERHF IMQVVCEATQ CPDTRVRVAA LQNLVKIMSL YYQYMETYMG PALFAITIEA MKSDIDEVAL QGIEFWSNVC DEEMDLAIEA SEAAEQGRPP EHTSKFYAKG ALQYLVPILT QTLTKQDEND DDDDWNPCKA AGVCLMLLAT CCEDDIVPHV LPFIKEHIKN PDWRYRDAAV MAFGCILEGP EPSQLKPLVI QAMPTLIELM KDPSVVVRDT AAWTVGRICE LLPEAAINDV YLAPLLQCLI EGLSAEPRVA SNVCWAFSSL AEAAYEAADV ADDQEEPATY CLSSSFELIV QKLLETTDRP DGHQNNLRSS AYESLMEIVK NSAKDCYPAV QKTTLVIMER LQQVLQMESH IQSTSDRIQF NDLQSLLCAT LQNVLRKVQH QDALQISDVV MASLLRMFQS TAGSGGVQED ALMAVSTLVE VLGGEFLKYM EAFKPFLGIG LKNYAEYQVC LAAVGLVGDL CRALQSNIIP FCDEVMQLLL ENLGNENVHR SVKPQILSVF GDIALAIGGE FKKYLEVVLN TLQQASQAQV DKSDYDMVDY LNELRESCLE AYTGIVQGLK GDQENVHPDV MLVQPRVEFI LSFIDHIAGD EDHTDGVVAC AAGLIGDLCT AFGKDVLKLV EARPMIHELL TEGRRSKTNK AKTLATWATK ELRKLKNQA
> 85% by SDS-PAGE.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
karyopherin subunit beta 1 , IMB1 , IPO1 , IPOB , Impnb , NTF97
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]