GeneTex
  • Country / Location Selection

United States (US)

Human Insulin protein, His tag (active)

Human Insulin protein, His tag (active). GTX67076-pro

Cat No. GTX67076-pro

Application

Functional Assay

Species

Human
Package
5 μg ($199)

APPLICATION

Application Note

Measured in a cell proliferation assay using MCF7 human breast cancer cells. The ED₅₀ for this effect is less or equal to 4 μg/ml.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

N-terminal His-Tag; Length: 109 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSFVNQHLC GSHLVEALYL VCGERGFFYT PKTRREAEDL QVGQVELGGG PGAGSLQPLA LEGSLQKRGI VEQCCTSICS LYQLENYCN

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Human Insulin protein, His tag (active). GTX67076-pro

GTX67076-pro Image

3 μg of GTX67076-pro Human Insulin protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

There are currently no references for Human Insulin protein, His tag (active) (GTX67076-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Human Insulin protein, His tag (active) (GTX67076-pro). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf