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Human JAK3 protein, His tag

Human JAK3 protein, His tag. GTX00160-pro
Human JAK3 protein, His tag. GTX00160-pro
Human JAK3 protein, His tag. GTX00160-pro

Cat. No. GTX00160-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($349)
See all JAK3 protein products

APPLICATION

Application Note

JAK3 (Tyrosine-protein kinase JAK3) is a non-receptor tyrosine kinase that involved in various processes such as cell growth, development, or differentiation. Besides, STAM2 has been identified as an interactor of JAK3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK3 and recombinant human STAM2. Briefly, JAK3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to STAM2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-JAK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of JAK3 and STAM2 was in a dose dependent manner.

Observed MW

32 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Trp716~Asp967 (NP_000206.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Janus kinase 3 , JAK-3 , JAK3_HUMAN , JAKL , L-JAK , LJAK

Background

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human JAK3 protein, His tag. GTX00160-pro

GTX00160-pro Functional Assay Image

Functional ELISA analysis of GTX00160-pro Human JAK3 protein which can bind immobilized STAM2 protein.

Human JAK3 protein, His tag. GTX00160-pro

GTX00160-pro Image

WB analysis of GTX00160-pro Human JAK3 protein.

Human JAK3 protein, His tag. GTX00160-pro

GTX00160-pro Image

SDS-PAGE analysis of GTX00160-pro Human JAK3 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 349