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Human Jagged 1 protein, human IgG1 Fc tag (active)

Human Jagged 1 protein, human IgG1 Fc tag (active). GTX65636-pro
Human Jagged 1 protein, human IgG1 Fc tag (active). GTX65636-pro

Cat No. GTX65636-pro

Application

Functional Assay

Species

Human
Package
10 μg ($289)

APPLICATION

Application Note

Inhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.

PROPERTIES

Form

Liquid

Buffer

PBS.

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (a.a. 1-1067) are fused at the C-terminus to the Fc portion of human IgG1.

Expression System

HEK293 cells

Purity

≥ 90% by SDS-PAGE.

Endotoxin

< 0.1 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

jagged canonical Notch ligand 1 , AGS , AGS1 , AHD , AWS , CD339 , DCHE , HJ1 , JAGL1

Database

  • Gene ID: 182 JAG1

Research Area

DATA IMAGES

Human Jagged 1 protein, human IgG1 Fc tag (active). GTX65636-pro

GTX65636-pro Image

Induction of Hes-1 with the treatment of GTX65636-pro Jagged 1 Fc fusion protein. A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of human Jagged1-Fc fusion protein as in indicated time points and each cell lysate was prepared and subjected to western blot by using human Hes1 antibody or GAPDH antibody.

Human Jagged 1 protein, human IgG1 Fc tag (active). GTX65636-pro

GTX65636-pro Image

Adipogenesis inhibition of 3T3L-1 cells. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0).The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (GTX65636-pro) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.

REFERENCE

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REVIEW

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SDS
PBS.pdf