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Human LIF protein, His tag (active)

Human LIF protein, His tag (active). GTX00201-pro
Human LIF protein, His tag (active). GTX00201-pro
Human LIF protein, His tag (active). GTX00201-pro

Cat. No. GTX00201-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($289)
See all LIF protein products

APPLICATION

Application Note

Leukemia Inhibitory Factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. To test the effect of LIF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard RPMI-1640 including various concentrations of recombinant human LIF. After incubated for 72h,   cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of TF-1 cells after incubation with LIF for 72h observed by inverted microscope.

Observed MW

26 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys2~Ala201 (NP_001244064.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIF interleukin 6 family cytokine , CDF , DIA , HILDA , MLPLI

Background

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Human LIF protein, His tag (active). GTX00201-pro

GTX00201-pro Functional Assay Image

Cell proliferation effect of GTX00201-pro Human LIF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated TF-1 cells cultured in RPMI-1640 for 72hrs.
(B) TF-1 cells cultured in RPMI-1640, stimulated with 0.5 ng/ml LIF for 72hrs.

Human LIF protein, His tag (active). GTX00201-pro

GTX00201-pro Image

SDS-PAGE analysis of GTX00201-pro Human LIF protein (active).

Human LIF protein, His tag (active). GTX00201-pro

GTX00201-pro Image

WB analysis of GTX00201-pro Human LIF protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 289