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Human LMTK3 protein, His and SUMO tag (active)

Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro
Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro
Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro
Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro

Cat. No. GTX00071-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all LMTK3 protein products

APPLICATION

Application Note

Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope. LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED₅₀ for this effect is approximately 6. 14-50 ng/ml.

Observed MW

46 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and SUMO-Tag; Leu133~Leu411

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lemur tyrosine kinase 3 , LMR3 , PPP1R101 , TYKLM3

Database

Research Area

DATA IMAGES

Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro

GTX00071-pro Functional Assay Image

Cell proliferation effect of GTX00071-pro Human LMTK3 protein (active).
(A) Unstimulated MCF-7 cells cultured in serum-free DMEM for 72hrs.
(B) MCF-7 cells cultured in serum-free DMEM, stimulated with 10 ng/ml LMTK3 for 72hrs.

Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro

GTX00071-pro Functional Assay Image

The dose effect curve of GTX00071-pro Human LMTK3 protein (active) on MCF-7 cells. After incubation of Human LMTK3 protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro

GTX00071-pro Image

SDS-PAGE analysis of GTX00071-pro Human LMTK3 protein (active).

Human LMTK3 protein, His and SUMO tag (active). GTX00071-pro

GTX00071-pro Image

WB analysis of GTX00071-pro Human LMTK3 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309