Human LOXL2 protein, His tag (0.05 μg, GTX136930-pro) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human LOXL2 protein, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (GTX136930-pro) (44.94-0.7 nM). Coated protein was probed with LOXL2 antibody (GTX105085) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.