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Human Lck protein, GST tag (active)

Human Lck protein, GST tag (active). GTX01273-pro

Cat No. GTX01273-pro

Application

Functional Assay

Species

Human
Package
50 μg ($339),
20 μg ($259)

APPLICATION

Application Note

1. The specific activity was determined to be 70 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
2. Measured by its ability to bind recombinant human CD3E protein in a functional ELISA.

Observed MW

80 kDa.

PROPERTIES

Form

Liquid

Buffer

50 mM Tris (pH 8.0), 100 mM NaCl, 0.5 mM GSH, 0.1 mM EGTA, 0.1 mM EDTA, 0.5 mM PMSF, 10% glycerol.

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

N-terminal GST tagged; Met1-Pro509 of Human Lck protein (NP_001036236.1)

Expression System

Baculovirus (Insect cells)

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IMD22,LSK,YT16,p56lck,pp58lck

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Human Lck protein, GST tag (active). GTX01273-pro

GTX01273-pro Image

SDS-PAGE of 5 μg GTX01273-pro Human Lck protein, GST tag (active).

REFERENCE

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Package List Price ($)
$ 339
$ 259