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Human M-CSF protein (active)

Comparison
Human M-CSF protein (active). GTX136909-pro
Human M-CSF protein (active). GTX136909-pro
Human M-CSF protein (active). GTX136909-pro

Cat. No. GTX136909-pro

Application

 Functional Assay

Species

 Human
Package
100 μg ($989)
See all M-CSF products
PRODUCT

Summary

M-CSF (also known as CSF1) is a cytokine that functions in the survival, proliferation, and differentiation of hematopoietic precursor cells. This bioactive recombinant human M-CSF is the 34 kDa form consisting of a disulfide-linked homodimer of two 159 a.a. subunits. Human M-CSF is active in murine systems, though the reverse is not the case.

APPLICATION

Application Note

The ED₅₀ was determined by a cell proliferation assay using murine M-NSF-60 cells cells is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.

Observed MW

17|28 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1 mg/ml. Lyophilized from 50mM PBS, 100mM NaCl, 5% Trehalose.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

Human full-length M-CSF protein (33-181 a.a. of NP_000748.4, signal peptide removed)

Expression System

E. Coli

Purity

>98%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Human M-CSF protein (active). GTX136909-pro

GTX136909-pro Functional Assay Image

Comparison

Serial dilutions of Human CSF1 protein (active) were added to murine M-NSF-60 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
*This product has been tested against the competitor and the biological activity is better than competitor.

Human M-CSF protein (active). GTX136909-pro

GTX136909-pro Image

1 μg of GTX136909-pro Human M-CSF protein (active) was analyzed using SDS-PAGE under reducing and stained with coomassie blue and captured by monochrome camera.

Human M-CSF protein (active). GTX136909-pro

GTX136909-pro Image

1 μg of GTX136909-pro Human M-CSF protein (active) was analyzed using SDS-PAGE under non-reducing and stained with coomassie blue and captured by monochrome camera.

REFERENCE

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REVIEW

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Package List Price ($)
$ 989