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Human MAGP1 protein (active)

Human MAGP1 protein (active). GTX00174-pro
Human MAGP1 protein (active). GTX00174-pro
Human MAGP1 protein (active). GTX00174-pro

Cat No. GTX00174-pro

Application Functional Assay
Species Human
Package
10 μg ($319)
APPLICATION

Application Note

Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was in a dose dependent manner.

Observed MW

38 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu6~Val162 (NP_001128719.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

microfibril associated protein 2,MAGP,MAGP-1,MAGP1

Background

Microfibrillar-associated protein 2 is a major antigen of elastin-associated microfibrils and a candidate for involvement in the etiology of inherited connective tissue diseases. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

DATA IMAGES
Human MAGP1 protein (active). GTX00174-pro

GTX00174-pro Functional Assay Image

Functional ELISA analysis of GTX00174-pro Human MAGP1 protein (active) which can bind immobilized FBN1 protein.

Human MAGP1 protein (active). GTX00174-pro

GTX00174-pro Image

SDS-PAGE analysis of GTX00174-pro Human MAGP1 protein (active).

Human MAGP1 protein (active). GTX00174-pro

GTX00174-pro Image

WB analysis of GTX00174-pro Human MAGP1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 319