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Human MASP2 protein (active)

Human MASP2 protein (active). GTX00122-pro
Human MASP2 protein (active). GTX00122-pro
Human MASP2 protein (active). GTX00122-pro

Cat No. GTX00122-pro

Application

Functional Assay

Species

Human
Package
10 μg ($309)

APPLICATION

Application Note

MASP2 (Mannan-binding lectin serine protease 2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. The preproprotein of MASP2 is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease, which is able to associate with MBL2. Thus, a functional binding ELISA assay was constructed to detect the association of rhMASP2 with MBL2. Briefly, rhMASP-2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05% Triton X-100 (pH 7.4). Duplicate samples of 100 μl were then transferred to MBL2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MASP-2 pAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MASP2 with MBL2 was in a dose dependent manner.

Observed MW

27 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile445~Ile683 (NP_006601.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mannan binding lectin serine peptidase 2 , MAP19 , MASP-2 , MASP1P1 , sMAP

Background

This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Human MASP2 protein (active). GTX00122-pro

GTX00122-pro Functional Assay Image

Functional ELISA analysis of GTX00122-pro Human MASP2 protein (active) which can bind immobilized MBL2 protein.

Human MASP2 protein (active). GTX00122-pro

GTX00122-pro Image

WB analysis of GTX00122-pro Human MASP2 protein (active).

Human MASP2 protein (active). GTX00122-pro

GTX00122-pro Image

SDS-PAGE analysis of GTX00122-pro Human MASP2 protein (active).

REFERENCE

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REVIEW

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