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Human MBL2 protein (active)

Human MBL2 protein (active). GTX00127-pro
Human MBL2 protein (active). GTX00127-pro
Human MBL2 protein (active). GTX00127-pro

Cat No. GTX00127-pro

Application Functional Assay
Species Human
Package
10 μg ($229)
APPLICATION

Application Note

MBL2 (Mannose-binding protein C) is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different microorganisms, therefore results in activation of the lectin pathway of the complement system. It has been proven that MASP-2 (Mannan-binding lectin serine protease 2) forms complexes with the pattern recognition molecules MBL2, triggers the activation of the complement system. Thus, a functional binding ELISA assay was constructed to detect the association of rhMBL2 with MASP2. Briefly, rhMBL2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05% Triton X-100 (pH 7.4). Duplicate samples of 100 μl were then transferred to MASP2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MBL2 mAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately.
The binding activity of MBL2 with MASP2 was in a dose dependent manner.

Observed MW

43 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Leu130~Ile248 (NP_000233.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mannose binding lectin 2,COLEC1,HSMBPC,MBL,MBL2D,MBP,MBP-C,MBP1,MBPD

Background

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human MBL2 protein (active). GTX00127-pro

GTX00127-pro Functional Assay Image

Functional ELISA analysis of GTX00127-pro Human MBL2 protein (active) which can bind immobilized MASP2 protein.

Human MBL2 protein (active). GTX00127-pro

GTX00127-pro Image

WB analysis of GTX00127-pro Human MBL2 protein (active).

Human MBL2 protein (active). GTX00127-pro

GTX00127-pro Image

SDS-PAGE analysis of GTX00127-pro Human MBL2 protein (active).

REFERENCE
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