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Human MCP1 / CCL2 protein (active)

Human MCP1 / CCL2 protein (active). GTX00415-pro
Human MCP1 / CCL2 protein (active). GTX00415-pro
Human MCP1 / CCL2 protein (active). GTX00415-pro

Cat No. GTX00415-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

MCP-1 (monocyte chemoattractant protein 1), also known as CCL2 (C-C motif chemokine 2), is a small cytokine that belongs to the CC chemokine family. MCP-1 has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MCP-1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 10⁶ cells/ml in RPMI-1640 with 0.5% FBS) and MCP-1 (25 and 50 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). MCP-1 is able to induce migration of THP-1 cells, and the optimum chemotactic concentration of MCP-1 is about 25 ng/ml.

Observed MW

15/17 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Gln24~Thr99 (NP_002973.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C motif chemokine ligand 2,GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES
Human MCP1 / CCL2 protein (active). GTX00415-pro

GTX00415-pro Functional Assay Image

The chemotactic effect of GTX00415-pro Human MCP1 / CCL2 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 25 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(C) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 50 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Human MCP1 / CCL2 protein (active). GTX00415-pro

GTX00415-pro Image

WB analysis of GTX00415-pro Human MCP1 / CCL2 protein (active).

Human MCP1 / CCL2 protein (active). GTX00415-pro

GTX00415-pro Image

SDS-PAGE analysis of GTX00415-pro Human MCP1 / CCL2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259