APPLICATION
Application Note
CCL13 (C-C motif chemokine 13) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, which belongs to the CC chemokine subfamily. It has been reported that CCL13 can induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CCL13 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶ cells/ml in RPMI-1640 with 0. 5% FBS) and CCL13 (50 ng/ml and 100 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly. CCL13 is able to induce migration of THP-1 cells.
Observed MW
13 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Phe17~Thr98 (NP_005399.1)
Expression System
E. coli
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
C-C motif chemokine ligand 13 , CKb10 , MCP-4 , NCC-1 , NCC1 , SCYA13 , SCYL1
Background
This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014]
Database
Research Area
DATA IMAGES
|
GTX00107-pro Functional Assay Image
The chemotactic effect of GTX00107-pro Human MCP4 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without CCL13 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and 50 ng/ml CCL13 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(C) THP-1 cells were seeded into the upper chambers and 100 ng/ml CCL13 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).
|
|
|
GTX00107-pro Image
SDS-PAGE analysis of GTX00107-pro Human MCP4 protein (active).
|
|
|
GTX00107-pro Image
WB analysis of GTX00107-pro Human MCP4 protein (active).
|
REFERENCE
There are currently no references for Human MCP4 protein, His tag (active) (GTX00107-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human MCP4 protein, His tag (active) (GTX00107-pro). Be the first to share your experience with this product.
