APPLICATION
Application Note
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶ cells/ml in RPMI-1640 with 0. 5% FBS) and MIP-1a (100 ng/ml, diluted in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). By counting migrated cells in low chamber at high magnification (×400) randomly. A mean of 41. 2 THP-1 cells/field migrated towards serum free RPMI-1640 medium with 100 ng/ml MIP-1a, while only 3. 6 THP-1 cells/field migrated towards serum free RPMI-1640 medium.
Observed MW
14 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Ser24~Ala92 (NP_002974.1)
Expression System
HEK293 cells
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
C-C motif chemokine ligand 3 , G0S19-1 , LD78ALPHA , MIP-1-alpha , MIP1A , SCYA3
Background
This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]
Database
Research Area
DATA IMAGES
|
GTX00096-pro Functional Assay Image
The chemotactic effect of GTX00096-pro Human MIP1 alpha protein (active). (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MIP-1 alpha was added in lower chamber, then cells in lower chamber were observed at low magnification (×40) after incubation for 5hrs. (B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 100 ng/ml MIP-1 alpha was added in lower chamber, then cells in lower chamber were observed at low magnification (×40) after incubation for 5hrs.
|
|
GTX00096-pro Image
WB analysis of GTX00096-pro Human MIP1 alpha protein (active).
|
|
GTX00096-pro Image
SDS-PAGE analysis of GTX00096-pro Human MIP1 alpha protein (active).
|
REFERENCE
There are currently no references for Human MIP1 alpha protein, His tag (active) (GTX00096-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human MIP1 alpha protein, His tag (active) (GTX00096-pro). Be the first to share your experience with this product.