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Human MIP1 alpha protein (active)

Human MIP1 alpha protein (active). GTX00096-pro
Human MIP1 alpha protein (active). GTX00096-pro
Human MIP1 alpha protein (active). GTX00096-pro

Cat No. GTX00096-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶ cells/ml in RPMI-1640 with 0. 5% FBS) and MIP-1a (100 ng/ml, diluted in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). By counting migrated cells in low chamber at high magnification (×400) randomly. A mean of 41. 2 THP-1 cells/field migrated towards serum free RPMI-1640 medium with 100 ng/ml MIP-1a, while only 3. 6 THP-1 cells/field migrated towards serum free RPMI-1640 medium.

Observed MW

14 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser24~Ala92 (NP_002974.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C motif chemokine ligand 3,G0S19-1,LD78ALPHA,MIP-1-alpha,MIP1A,SCYA3

Background

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES
Human MIP1 alpha protein (active). GTX00096-pro

GTX00096-pro Functional Assay Image

The chemotactic effect of GTX00096-pro Human MIP1 alpha protein (active).
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MIP-1 alpha was added in lower chamber, then cells in lower chamber were observed at low magnification (×40) after incubation for 5hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 100 ng/ml MIP-1 alpha was added in lower chamber, then cells in lower chamber were observed at low magnification (×40) after incubation for 5hrs.

Human MIP1 alpha protein (active). GTX00096-pro

GTX00096-pro Image

SDS-PAGE analysis of GTX00096-pro Human MIP1 alpha protein (active).

Human MIP1 alpha protein (active). GTX00096-pro

GTX00096-pro Image

WB analysis of GTX00096-pro Human MIP1 alpha protein (active).

REFERENCE
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REVIEW
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