APPLICATION
Application Note
Macrophage inflammatory protein-1β (MIP-1β), also known as CCL4 is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1β is a kind of chemotactic cytokine. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MIP-1β on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100 μl cell suspension, 5x10⁵ cell/ml in RPMI-1640 with FBS free) and recombinant human MIP-1β (0. 0001 ng/ml, 0. 001 ng/ml, 0. 1 ng/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×200) randomly. The recombinant human MIP-1β is able to induce migration of Jurkat cells.
Observed MW
14 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Ala24~Asn92 (NP_002975.1)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
C-C motif chemokine ligand 4 , ACT2 , AT744.1 , G-26 , HC21 , LAG-1 , LAG1 , MIP-1-beta , MIP1B , MIP1B1 , SCYA2 , SCYA4
Background
The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]
Database
Research Area
DATA IMAGES
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GTX00279-pro Functional Assay Image
The chemotactic effect of GTX00279-pro Human MIP1 beta protein (active).
Upper: (A) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 without MIP-1 beta was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2hrs.
(B) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 with 0.01 ng/ml MIP-1 beta was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2hrs.
Lower: The number of migrated cells were counted at high magnification (×200) randomly (five fields for each filter).
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GTX00279-pro Image
WB analysis of GTX00279-pro Human MIP1 beta protein (active).
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GTX00279-pro Image
SDS-PAGE analysis of GTX00279-pro Human MIP1 beta protein (active).
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REFERENCE
There are currently no references for Human MIP1 beta protein, His tag (active) (GTX00279-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human MIP1 beta protein, His tag (active) (GTX00279-pro). Be the first to share your experience with this product.
