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Human MIP3 alpha protein (active)

Human MIP3 alpha protein (active). GTX00192-pro
Human MIP3 alpha protein (active). GTX00192-pro
Human MIP3 alpha protein (active). GTX00192-pro

Cat No. GTX00192-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

Macrophage Inflammatory Protein 3 Alpha (MIP3a) also known as Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. MIP3a is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. It is expressed in several tissues such as peripheral blood lymphocytes, lymph nodes, liver, appendix, fetal lung. Expression of MIP3a can be induced by microbial factors such as lipopolysaccharide (LPS), and inflammatory cytokines such as tumor necrosis factor and interferon-γ, and down-regulated by IL-10. Besides, Transforming Growth Factor Beta 3 (TGFb3) has been identified as an interactor of MIP3a, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIP3a and recombinant human TGFb3. Briefly, MIP3a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TGFb3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MIP3a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MIP3a and TGFb3 was in a dose dependent manner.

Observed MW

13 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala27~Met96 (NP_001123518.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C motif chemokine ligand 20 , CKb4 , Exodus , LARC , MIP-3-alpha , MIP-3a , MIP3A , SCYA20 , ST38

Background

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES
Human MIP3 alpha protein (active). GTX00192-pro

GTX00192-pro Functional Assay Image

Functional ELISA analysis of GTX00192-pro Human MIP3 alpha protein (active) which can bind immobilized TGFb3 protein.

Human MIP3 alpha protein (active). GTX00192-pro

GTX00192-pro Image

WB analysis of GTX00192-pro Human MIP3 alpha protein (active).

Human MIP3 alpha protein (active). GTX00192-pro

GTX00192-pro Image

SDS-PAGE analysis of GTX00192-pro Human MIP3 alpha protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289