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Human NEU1 protein (active)

Human NEU1 protein (active). GTX00129-pro
Human NEU1 protein (active). GTX00129-pro
Human NEU1 protein (active). GTX00129-pro

Cat No. GTX00129-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was in a dose dependent manner.

Observed MW

26 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala141~His286 (NP_000425.1)

Expression System

293F cell

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neuraminidase 1,NANH,NEU,SIAL1

Background

The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human NEU1 protein (active). GTX00129-pro

GTX00129-pro Functional Assay Image

Functional ELISA analysis of GTX00129-pro Human NEU1 protein (active) which can bind immobilized CTSA protein.

Human NEU1 protein (active). GTX00129-pro

GTX00129-pro Image

WB analysis of GTX00129-pro Human NEU1 protein (active).

Human NEU1 protein (active). GTX00129-pro

GTX00129-pro Image

SDS-PAGE analysis of GTX00129-pro Human NEU1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309