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Human NEU1 protein (active)

Human NEU1 protein (active). GTX00130-pro
Human NEU1 protein (active). GTX00130-pro
Human NEU1 protein (active). GTX00130-pro

Cat No. GTX00130-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was in a dose dependent manner.

Observed MW

46 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala47~Leu415 (NP_000425.1)

Expression System

293F cell

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neuraminidase 1 , NANH , NEU , SIAL1

Background

The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human NEU1 protein (active). GTX00130-pro

GTX00130-pro Functional Assay Image

Functional ELISA analysis of GTX00130-pro Human NEU1 protein (active) which can bind immobilized CTSA protein.

Human NEU1 protein (active). GTX00130-pro

GTX00130-pro Image

SDS-PAGE analysis of GTX00130-pro Human NEU1 protein (active).

Human NEU1 protein (active). GTX00130-pro

GTX00130-pro Image

WB analysis of GTX00130-pro Human NEU1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309