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Human NG2 protein (active)

Human NG2 protein (active). GTX00208-pro
Human NG2 protein (active). GTX00208-pro
Human NG2 protein (active). GTX00208-pro

Cat No. GTX00208-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

Melanoma-associated chondroitin sulfate proteoglycan (MCSP) is a chondroitin sulfate proteoglycan in humans. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. Besides, Galectin-8 (GAL8) has been identified as an interactor of MCSP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MCSP and recombinant human GAL8. Briefly, MCSP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GAL8-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MCSP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MCSP and GAL8 was in a dose dependent manner.

Observed MW

29 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser1705~Ile1942 (NP_001888.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chondroitin sulfate proteoglycan 4,HMW-MAA,MCSP,MCSPG,MEL-CSPG,MSK16,NG2

Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human NG2 protein (active). GTX00208-pro

GTX00208-pro Functional Assay Image

Functional ELISA analysis of GTX00208-pro Human NG2 protein (active) which can bind immobilized GAL8 protein.

Human NG2 protein (active). GTX00208-pro

GTX00208-pro Image

WB analysis of GTX00208-pro Human NG2 protein (active).

Human NG2 protein (active). GTX00208-pro

GTX00208-pro Image

SDS-PAGE analysis of GTX00208-pro Human NG2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289