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Human NG2 protein (active)

Human NG2 protein (active). GTX00208-pro
Human NG2 protein (active). GTX00208-pro
Human NG2 protein (active). GTX00208-pro

Cat No. GTX00208-pro

Application

Functional Assay

Species

Human
Package
10 μg ($289)

APPLICATION

Application Note

Melanoma-associated chondroitin sulfate proteoglycan (MCSP) is a chondroitin sulfate proteoglycan in humans. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. Besides, Galectin-8 (GAL8) has been identified as an interactor of MCSP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MCSP and recombinant human GAL8. Briefly, MCSP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GAL8-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MCSP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MCSP and GAL8 was in a dose dependent manner.

Observed MW

29 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser1705~Ile1942 (NP_001888.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

chondroitin sulfate proteoglycan 4 , HMW-MAA , MCSP , MCSPG , MEL-CSPG , MSK16 , NG2

Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human NG2 protein (active). GTX00208-pro

GTX00208-pro Functional Assay Image

Functional ELISA analysis of GTX00208-pro Human NG2 protein (active) which can bind immobilized GAL8 protein.

Human NG2 protein (active). GTX00208-pro

GTX00208-pro Image

WB analysis of GTX00208-pro Human NG2 protein (active).

Human NG2 protein (active). GTX00208-pro

GTX00208-pro Image

SDS-PAGE analysis of GTX00208-pro Human NG2 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 289