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Human NGF protein (active)

Human NGF protein (active). GTX00097-pro
Human NGF protein (active). GTX00097-pro
Human NGF protein (active). GTX00097-pro
Human NGF protein (active). GTX00097-pro

Cat No. GTX00097-pro

Application

Functional Assay

Species

Human
Package
10 μg ($249)

APPLICATION

Application Note

Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham'SF12K containing 5% fetal calf serum and 10% horse serum on  polylysine or collagen coated plates. When cells reached log phase growth, fresh medium was added together with 10 ng/ml of NGF, then cells were observed by inverted microscope.
NGF is also involved in pathways besides those regulating the life cycle of neurons. Besides, Alpha-2-Macroglobulin (a2M) has been identified as an interactor of NGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human NGF and recombinant human a2M. Briefly, NGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to a2M-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of NGF and a2M was in a dose dependent manner.

Observed MW

30/33 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu19~Arg121+DDDDK+Ser122~Arg239 (NP_002497.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nerve growth factor , Beta-NGF , HSAN5 , NGFB

Background

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human NGF protein (active). GTX00097-pro

GTX00097-pro Functional Assay Image

Functional ELISA analysis of GTX00097-pro Human NGF protein (active) which can bind immobilized a2M protein.

Human NGF protein (active). GTX00097-pro

GTX00097-pro Functional Assay Image

Cell differentiation effect of GTX00097-pro Human NGF protein (active).
(A) Unstimulated PC-12 cells cultured in Ham's F12K containing 5% fetal calf serum and 10% horse serum on collagen coated plates.
(B) PC-12 cells cultured in Ham's F12K containing 5% fetal calf serum and 10% horse serum on collagen coated plates, stimulated with 10 ng/ml NGF for 6 days.

Human NGF protein (active). GTX00097-pro

GTX00097-pro Image

WB analysis of GTX00097-pro Human NGF protein (active).

Human NGF protein (active). GTX00097-pro

GTX00097-pro Image

SDS-PAGE analysis of GTX00097-pro Human NGF protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 249