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Human NPEPPS protein (active)

Human NPEPPS protein (active). GTX00222-pro
Human NPEPPS protein (active). GTX00222-pro
Human NPEPPS protein (active). GTX00222-pro

Cat No. GTX00222-pro

Application Functional Assay
Species Human
Package
10 μg ($249)
APPLICATION

Application Note

Puromycin Sensitive Aminopeptidase (PSA) also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is found at high levels in the urine when there are kidney problems. PSA has been proposed to function in a variety of processes, including metabolism of neuropeptidase, regulation of the cell cycle, and hydrolysis of proteasomal products to amino acids. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of PSA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PSA and recombinant human PDIA3. Briefly, PSA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PSA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of PSA and PDIA3 was in a dose dependent manner.

Observed MW

25 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly584~Gln793 (NP_001317186.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aminopeptidase puromycin sensitive , AAP-S , MP100 , PSA

Background

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Human NPEPPS protein (active). GTX00222-pro

GTX00222-pro Functional Assay Image

Functional ELISA analysis of GTX00222-pro Human NPEPPS protein (active) which can bind immobilized PDIA3 protein.

Human NPEPPS protein (active). GTX00222-pro

GTX00222-pro Image

WB analysis of GTX00222-pro Human NPEPPS protein (active).

Human NPEPPS protein (active). GTX00222-pro

GTX00222-pro Image

SDS-PAGE analysis of GTX00222-pro Human NPEPPS protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 249