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Human Neuregulin-1 protein (active)

Human Neuregulin-1 protein (active). GTX00417-pro
Human Neuregulin-1 protein (active). GTX00417-pro
Human Neuregulin-1 protein (active). GTX00417-pro
Human Neuregulin-1 protein (active). GTX00417-pro

Cat No. GTX00417-pro

Application Functional Assay
Species Human
Package
10 μg ($329)
APPLICATION

Application Note

NRG1 (neuregulin-1) is a membrane glycoprotein that belongs to the neuregulin family and act on the EGFR family of receptors. It mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. It is reported that by binding to HER3 receptor, NRG1 mediates downstream signaling pathways, leading to multiple effects including growth, proliferation, decreased apoptosis, cellular migration and angiogenesis. Thus, proliferation assay of NRG1 was conducted using MCF-7 cells. Briefly, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of NRG1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10 μl of CCK-8 solution was added to each well of the plate, the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. The dose-effect curve of NRG1 showed it significantly promoted cell proliferation of MCF-7 cells, and the ED₅₀ for this effect is typically 5.240 to 9.112 ng/ml.

Observed MW

27 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu20~His242 (NP_001153467.1)

Expression System

E. coli

Purity

> 98%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neuregulin 1 , ARIA , GGF , GGF2 , HGL , HRG , HRG1 , HRGA , MST131 , MSTP131 , NDF , NRG1-IT2 , SMDF

Background

The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES
Human Neuregulin-1 protein (active). GTX00417-pro

GTX00417-pro Functional Assay Image

Cell proliferation effect of GTX00417-pro Human Neuregulin-1 protein (active) on MCF-7 cells. After incubation of Human Neuregulin-1 protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8). The ED50 for this effect is typically 5.240 to 9.112 ng/ml.

Human Neuregulin-1 protein (active). GTX00417-pro

GTX00417-pro Functional Assay Image

Cell proliferation effect of GTX00417-pro Human Neuregulin-1 protein (active).
(A) Unstimulated MCF-7 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 10 ng/ml NRG1 for 72hrs.

Human Neuregulin-1 protein (active). GTX00417-pro

GTX00417-pro Image

WB analysis of GTX00417-pro Human Neuregulin-1 protein (active).

Human Neuregulin-1 protein (active). GTX00417-pro

GTX00417-pro Image

SDS-PAGE analysis of GTX00417-pro Human Neuregulin-1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 329